Nah, Vista accesses the hard drive like crazy for me after quite some time from booting. It was like always solid after I installed it but now it's died down after a few months.
Are you using 32bit or 64bit?
My friend has the DS3L motherboard and Vista x64 and the P35 drivers installed fine (from Gigabyte), and he has a NVIDIA card so can't judge ATI drivers.
Maybe try installing this "2005 Windows Redistributal" by itself? Download it from MS or whatever. Also make sure you have all the Windows updates.
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